Handbook of RNA Biochemistry (Hardcover (Trade Cloth))

Buy Handbook of RNA Biochemistry (Hardcover (Trade Cloth))

Buy Handbook of RNA Biochemistry (Hardcover (Trade Cloth))

Handbook of RNA Biochemistry (Hardcover (Trade Cloth)) Product Description

Handbook of RNA Biochemistry Covering all topics relating to current RNA research laboratory techniques, this text looks at both the side-by-side treatment of specialised methods along with more basic biochemical techniques. This all-in-one compendium of current laboratory techniques for the study of RNA brings together numerous novel methods that were hitherto only available through the primary literature. The editors - themselves experienced RNA researchers - have gathered expert authors from the US and Europe for the ultimate in everyday working knowledge. In more than 300 detailed protocols, all the techniques covered are treated with the user in mind, including practical advice and an invaluable troubleshooting section.The broad scope of the book allows the side-by-side treatment of specialized methods with basic biochemical techniques for the isolation, characterization and functional analysis of RNA. All pertinent laboratory methods are presented in detail, including RNA synthesis, modification and ligation Sequence and structure determination of RNA Isolation and purification of ribonucleoproteins RNA crystallization Single molecule studies on RNA RNA imaging in vivo RNA bioinformatics Modeling and prediction of RNA structure RNA microarrays Aptamer libraries and SELEX technology RNA interferenceA guide to successful experimentation for molecular biologists, biochemists and biotechnologists and for every researcher experimenting with RNA. RNA SYNTHESIS Enyzmatic synthesis, ligation and modification Chemical synthesis STRUCTURE DETERMINATION Sequence determination Secondary and tertiary structure determination Footprinting techniques Identification of interacting proteins and nucleic acids Nucleotide analog interference mapping Atomic structure determination Spectroscopic studies of RNA structure GENOMICS BIOINFORMATICS Phylogenetic analysis Structure prediction Molecular dynamics simulation RNA mining and RNomics ANALYSIS OF RNA FUNCTION Isolation and purification of RNA-binding proteins Genetic analysis of RNA-binding proteins Three-hybrid analysis to identify RNA-binding proteins Analysis of mRNA splice variants Confocal microscopy studies of intracellular localization In vitro selection of ribozymes and aptamers RNA INTERFERENCE Strategies for in vivo RNAi studies (partial contents) This all-in-one compendium of current laboratory techniques for the study of RNA brings together numerous novel methods that were hitherto only available through the primary literature. The editors - themselves experienced RNA researchers - have gathered expert authors from the US and Europe for the ultimate in everyday working knowledge. To this end, all the techniques covered are treated with the user in mind, including detailed practical advice and an invaluable troubleshooting section. The broad scope of the book allows the side-by-side treatment of specialized methods with basic biochemical techniques for the isolati

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