Integrated Genomics A Discovery-Based Laboratory Course (Hardcover (Trade Cloth))

Buy Integrated Genomics A Discovery-Based Laboratory Course (Hardcover (Trade Cloth))

Buy Integrated Genomics A Discovery-Based Laboratory Course (Hardcover (Trade Cloth))

Integrated Genomics A Discovery-Based Laboratory Course (Hardcover (Trade Cloth)) Product Description

Integrated Genomics A Discovery-Based Laboratory Course This text introduces the excitement of discovery to the basic molecular biology laboratory. Utilising up-to-date molecular biology protocols and a basic experimental design, this text offers experience with three different model systems. It is aimed at those pursuing careers in biomedicine, pharmacy, and biotechnology. Integrated Genomics A Discovery-Based Laboratory Course introduces the excitement of discovery to the basic molecular biology laboratory. Utilizing up-to-date molecular biology protocols and a basic experimental design, this text offers experience with three different model systems. Students will become familiar with the simplicity and power of single-celled organisms, Escherichia coli and Saccharomyces cerevisiae, as they search for genes that interact and function within the nematode Caenorhabditis elegans, Incorporated throughout the course are exercises designed to offer students familiarity with the wealth of bioinformatics data that can be accessed on the World Wide Web. Following completion of interaction studies within the yeast, the course is designed to allow students to examine the functional consequences of reducing a genes function within the multicellular worm that is both simple and inexpensive to maintain within a laboratory. The inclusion of alternative experiments allow for flexibility in determining the ending date or goal of the laboratory, as well as working within the available budget and resources of most any classroom environment. Further striking features of this title are An accompanying Web site providing PowerPoint slides, plus links to the internet, and regular updates as bioinformatics databases evolve and methods improve. www.wiley.comgocaldwell Inclusion of modern genomicproteomic technologies such as the yeast two-hybrid system and RNAi Detailed experimental protocols and easy access to instructional materials This discovery-based laboratory course provides excellent practical training for those pursuing career paths inbiomedicine, pharmacy, and biotechnology. Preface. Author biographies. Acknowledgments. List of figures. 1 Introduction to basic laboratory genetics. 1.1 Transferring and handling C. elegans. 1.2 Introduction to laboratory genetics. 2 Gene expression analysis using transgenic animals. 2.1 Transgenic gene expression analysis in C. elegans lacZ staining. 2.2 Transgenic gene expression analysis in C. elegans GFP analysis. 3 Creation and testing of transgenic yeast for use in protein-protein interaction screening. 3.1 Small-scale transformation of S. cerevisiae. 3.2 Transformation of S. cerevisiae to test for non-specific interaction. 3.3 Assaying for protein-protein interaction by reporter gene expression. 4 Yeast two-hybrid screening. 4.1 Protein-protein interaction screening of a C. elegans cDNA library. 4.2 Assaying for protein-protein interaction by reporter gene expression. 5 Isolation and identification of interac

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