RNA Methodologies A Laboratory Guide for Isolation and Characterization (3rd Edition) (Hardcover (Trade Cloth))

Buy RNA Methodologies A Laboratory Guide for Isolation and Characterization (3rd Edition) (Hardcover (Trade Cloth))

Buy RNA Methodologies A Laboratory Guide for Isolation and Characterization (3rd Edition) (Hardcover (Trade Cloth))

RNA Methodologies A Laboratory Guide for Isolation and Characterization (3rd Edition) (Hardcover (Trade Cloth)) Product Description

RNA Methodologies A Laboratory Guide for Isolation and Characterization (3rd Edition) This laboratory guide represents a growing collection of tried, tested and optimised laboratory protocols for the isolation and characterisation of eukaryotic RNA, with lesser emphasis on the characterisation of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story. The isolation of biologically competent and chemically stable RNA is central to molecular biology. There remains an academic imperative to unify the many complex facets of the characterizations of cellular biochemistry with particular regards to the use of RNA as a parameter of gene expression. This laboratory guide is a growing collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. The focus herein is how these methodologies can be used in the study of transcriptional- and post-transcriptional regulation of gene expression. KEY FEATURES New sections describing RNA isolation, subtraction-suppression PCR, high-throughput methods, bioinformatics, and RNAi Explains proper handling, storage, and manipulation of RNA An expanded section on RT-PCR, RACE, and related techniques Trouble-shooting options are incorporated throughout the text This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30 new material, including chapters on the more recent technologies of RNA interference including RNAi Microarrays Bioinformatics. It also includes new sections on new and improved RT-PCR techniques innovative 5 and 3 RACE techniques subtractive PCR methods methods for improving cDNA synthesis. Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center Includes classic and contemporary techniques Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects The expanded third edition of this highly successful laboratory manual presents new technologies in RNA interference RNA and the Cellular Biochemistry Revisited Transcription and the Organization of Eukaryotic Genes Messenger RNA Resilient Ribonucleases RNA Isolation Strategies The Truth About Tissues Isolation of Polyadenylated RNA Quality Control for RNA Preparations Dot Blot Analysis Electrophoresis of RNA Photodocumentation and Image Analysis Northern Analysis Nucleic Acid Probe Technology Practical Nucleic Acid Hy

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